|Microbiome of the Uterus in Women With Unsuccessful in Vitro Fertilization Attempts|
|Barinova Viktoriya Vladislavovna1, 2, Kuznetsova Natalya Borisovna1, 2, Bushtyreva Irina Olegovna2, Sokolova Kristina Mikhailovna2, Polev Dmitry Evgenievich3, Aseev Mihail Vladimirovich3, Dudurich Vasilisa Valerievna3|
|1Federal State Budget Establishment “Rostov-on-Don State Medical University” of Ministry of Health, Rostov-on-Don, Russia
2Clinic of Professor Bushtyreva LLC, Rostov-on-Don, Russia
3Medical Genetics Center “Serbalab”, St. Petersburg, V.O., Bolshoy Avenue, 90, Building 2, Russia
IJWHR 2020; 8: 423-427
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Keywords : Uterine microbiome, Infertility, Lactobacillus, IVF failure
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Objectives: This study aimed to investigate the peculiarities of the uterine microbiome in the case of infertility and repeated in vitro fertilization (IVF) failures. Accordingly, the microbiome of the uteri of 22 women with 2 or more IVF failures (first group) and 20 healthy women (second group) was analyzed in this study.
Materials and Methods: The samples of uterine microbiomes were collected 7 days after the luteinizing hormone elevation, which was determined by the urine test. All measures were taken to avoid sample contamination. Finally, the massively parallel sequencing of the 16S ribosomal RNA gene was done in every uterine sample.
Results: The analysis of the mean relative abundance of various microorganisms in the uterine microbiome showed that women with infertility had higher microbiological diversity and variability compared to healthy women. Eventually, the mean relative abundance of the Lactobacillus genus comprised 34.4% and 63.0% in the first and second groups, respectively.
Conclusion: In general, the presence of Lactobacillus in the uterine microbiome could be considered a favorable factor for good reproductive outcomes and successful IVF programs.
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