International Journal of Women's Health and Reproduction Sciences

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Jul 2024, Vol 12, Issue 3

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Original Article
Expression of MCM6 in the Sperm of Teratozoospermia Infertile Males
Mina Moayeri¹, Shiva Irani¹, Marefat Ghaffari Novin², Iman Salahshourifar¹, Mohammad Salehi^3,4
¹Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran ²Department of Biology and Anatomical Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran ³Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ⁴Department of Biotechnology, School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences, Tehran, Iran
DOI: 10.15296/ijwhr.2023.8004 Viewed : 781 times Downloaded : 489 times. Keywords : Infertility, Teratozoospermia, DNA replication, MCM6, and DNA integrity
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Abstract

Objectives: MCM6 is an essential member of the MCM family and a key component of the DNA replication machinery that regulates cell cycle progression and genome stability. Previous studies suggested that genome integrity is an important factor in male infertility. Therefore, reducing the expression of this gene may be effective in male infertility, which has not been investigated yet. We aimed to evaluate the expression level of this gene in the sperm of infertile males with teratozoospermia and compare it with control individuals. Material and Methods: In this study, we included a total of 20 fertile healthy males in the control group and 40 individuals with teratozoospermia in the case group. After cDNA synthesis and total RNA extraction, relative quantification was carried out. The normalizer gene utilized was GAPDH, and the fold change was computed using the 2−ΔΔCt formula. Considering a significance threshold of P < 0.05, a t-test was employed to compare the expression levels between the case and control groups. Results: According to our findings, teratozoospermia males had significantly lower levels of MCM6 (logFC=0.065) than controls. Moreover, the DNA fragmentation index of normal individuals was 16.00 ± 3.00, while this index was 33.72 ± 5.098 for teratozoospermia patients. Conclusions: Since MCM6 downregulation is associated with teratozoospermia, it might be proposed as a possible candidate gene for the progression of this condition. Also, it could be considered as a potential diagnostic marker and have other therapeutic uses.