Login Contact Us
Jan 2019, Vol 7, Issue 1
Advanced Search
Title
Authors
Keyword
Poll
How do you find the scientific quality of the published articles on our web site?


Original Article
The Effect of Implant Origin Differences on Peritoneal Endometriosis in An Endometriosis Mouse Model
Sutrisno Sutrisno1, Sri Andarini1, I Wayan Arsana Wiyasa1, Umi Kulsum1, Noerhamdani Noerhamdani1, Hidayat Suyuti1, Hendy Hendarto1
1Department of Obstetrics and Gynecology, Faculty of Medicine, Brawijaya University, Malang 65145, East Java, Indonesia

IJWHR 2019; 7: 034-040
DOI: 10.15296/ijwhr.2019.06
Viewed : 3228 times
Downloaded : 2874 times.

Keywords : Endometriosis, Granuloma mass, Implant area, Peritoneal damage
Full Text(PDF) | Related Articles
Abstract

Objectives: This study aimed to observe the difference in the area of endometriosis lesions and the histopathology of inflammatory cells and granuloma masses in an endometriosis mouse model treated with endometrial cell implants, endometrioma capsules, and adenomyosis tissue.

Materials and Methods: This is an experimental study with posttest-only research design which was conducted with the control group. Thirty-two mice (Mus musculus) were injected with 0.2 mL/mice cyclosporin A and then were divided into three groups which were injected with endometrial tissue from the uterine cavity (group A), endometriosis from endometrioma capsule (group B), and endometriosis from adenomyosis (group C). The injection was done slowly into the peritoneal cavity, 0.1 mL each, and followed by intramuscularly Ethinyl estradiol, 0.2 ?G/mice. On the 15th days, mice were dissected to observe the peritoneal endometriosis implant and microscopic examination with hematoxylin-eosin (HE) staining to determine the inflammatory cell infiltration and mass granuloma presence. Data were analyzed using SPSS, version 19.

Results: The study obtained that the area of implanted endometriosis lesions in group C covered a larger area of endometriosis implants than other groups (P < 0.05). The peritoneal damage in group C was the most severe based on the Klopfleisch method (P < 0.05), with mass granuloma and massive infiltration of inflammatory cells and fibrous connective tissue formation occurring in muscle tissue.

Conclusions: The implantation of adenomyosis cell tissue is the best method to develop mice model of endometriosis based on its inflammatory infiltration, the extent of lesion implant, and granuloma mass

Cite By, Google Scholar

Google Scholar

Articles by Sutrisno S
Articles by Andarini S
Articles by Kulsum U
Articles by Noerhamdani N
Articles by Suyuti H
Articles by Hendarto H

PubMed

Articles by Sutrisno Sutrisno
Articles by Sri Andarini
Articles by Umi Kulsum
Articles by Hidayat Suyuti
Articles by Hendy Hendarto

Submit Paper
Online Submission System
IJWHR ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Published Article Statistics